基于1H-NMR技术的产后卵巢静止奶牛乳清和血清代谢谱分析

doi:10.11843/j.issn.0366-6964.2018.12.009

Abstract

Abstract:

The study aimed to use metabolomics techniques to screen the differential metabolites in whey and serum of ovarian quiescence cows and to determine the relationship between differential metabolites and the ovary quiescence of cows. In the study, 14 estrus cows and 14 ovarian quiescence cows from 45-60 d postpartum were selected for testing. The whey and serum of cows in the two groups were tested using ¹H-NMR technique. Differential metabolites in whey and serum of cows between the two groups were screened by multivariate statistical analysis and univariate analysis. Bioinformatics analysis was performed to elucidate the relationship between metabolic pathways involved in differential metabolites and ovarian quiescence of dairy cows. The results showed that 13 differential metabolites in cows between the two groups were screened in whey and serum, respectively. Compared with estrus cows, the contents of 6 differential metabolites increased in the whey of ovarian quiescence cows, which were succinate, creatine phosphate, glycine, myo-inositol, glycolate,orotate; and the contents of 7 differential metabolites decreased, which were alanine, creatinine, O-phosphorylcholine, lactose, taurine, galactose and glucose-1-phosphate. The contents of 4 differential metabolites increased in the serum of ovarian quiescence cows, which were β-hydroxybutyrate, acetate, glutamine, glycine; and the contents of 9 differential metabolites reduced, which were alanine, succinate, citrate, creatinine, O-phosphocholine, glucose, myo-inositol, tyrosine and histidine. The differential metabolites in whey of cows between the two groups were mainly involved in the metabolism of taurine and hypotaurine, the metabolism of galactose, and the biosynthesis of primary bile acids. The differential metabolites in serum were mainly involved in alanine, aspartate and glutamate metabolism, glyoxylic acid and dicarboxylic acid metabolism, citric acid cycle, phenylalanine, tyrosine and tryptophan biosynthesis.In this study, the metabolic profiles and differential metabolites in whey and serum of ovarian quiescence cows were obtained. The results showed that the main abnormal pathways of glucose, lipid and amino acid metabolism played a role in postpartum ovarian quiescence in dairy cows, which provided a new direction for further studying the mechanism and prevention strategy of postpartum ovarian quiescence in dairy cows.

CLC Number:

S823.3

FAN Zi-ling, SONG Yu-xi, ZHANG Jiang, ZHAO Chang, BAI Yun-long, SUN Shu-han, XIA Cheng. Analysis of Metabolic Profiles of Whey and Serum in Postpartum Dairy Cows with Ovary Quiescence Based on ¹H-NMR[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(12): 2602-2611.

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Analysis of Metabolic Profiles of Whey and Serum in Postpartum Dairy Cows with Ovary Quiescence Based on ¹H-NMR

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Analysis of Metabolic Profiles of Whey and Serum in Postpartum Dairy Cows with Ovary Quiescence Based on 1H-NMR

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Analysis of Metabolic Profiles of Whey and Serum in Postpartum Dairy Cows with Ovary Quiescence Based on ¹H-NMR